Retrospective clinical research. University hospital, infertility clinic. Patients with one or two follicles, between the ages of 20 and 40, whose infertility period was less than 6 years and the injected total motile sperm count was more than 10 million. Intrauterine insemination IUI is used in combination with controlled ovarian hyperstimulation COH in treating couples with unexplained infertility, mild-moderate male factor, stage endometriosis, anovulation or cervical factor 1 , 2. As a changeable and customizable factor, sperm preparation techniques also have the potential of affecting the IUI results. There are many studies exploring the effects of sperm preparation techniques and the temperature of the environment in which sperms are preserved until insemination on the sperm parameters.
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IUI Lab Setup | A.R.T. Lab setup
Once thawed, the contents of the vial will be liquid. Warm the vial to body temperature in your hands or under your arm before inseminating. And please do not store the vial in your refrigerator or freezer prior to thawing—a standard freezer is not sufficiently cold to keep sperm frozen. If you are planning on inseminating at home we will provide you with a needle-less syringe for vaginal insemination. Remove the syringe from the plastic wrap, and pump the plunger in and out a couple of times to break the seal. Shake the thawed vial well before removing its lid, then insert the tip of the syringe into the vial and draw the semen into the syringe by pulling the plunger up be careful not to pull the plunger all the way out of the syringe. Do not clean the syringe before using it; even rinsing the syringe with plain water could kill sperm.
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Getting Pregnant With IUI: What You Need to Know
I had made the mistake of going down a Google rabbit hole, plumbing the depths of parenting forums for any personal stories about intrauterine insemination — especially the negative ones. I wanted to mentally prepare myself. Would it hurt? Would there be a lot of cramping afterward? Could something unexpectedly go awry?
Sperm washing is the process in which individual sperms are separated from the semen. Washed sperm is used in artificial insemination using the intrauterine insemination IUI technique and in in vitro fertilization IVF. It may also be used to decrease the risk of HIV transmission by an HIV-positive male, in which case the washed sperm is injected into a female using an artificial insemination technique.